Research Paper Example | Topics and Well Written Essays - 2000 words - 2

Exploration Paper Example Be that as it may, the quantity of adolescent pregnancy in sub-Saharan countries drives the overall pack. In actuality, the adolescents who get themselves pregnant face extra clinical issues for their infants. For the most part, these infants will in general be under weight during birth because of the periods of their moms. Then again, high school pregnancies will in general draw impact from various components. To start with, the social issue that may incorporate training level is a vital impact. Also, the financial status from which a youngster originates from is a factor that achieves this cultural issue. In light of these realities, this paper will show how high school pregnancies will in general impact youth training according to various companion evaluated articles as sources. Individual reflection Over the years, high school pregnancies have incredibly influenced the learning progress of adolescents and youthful grown-ups. In my view, society gives negligible consideration to t he dangers engaged with the intermittence of instruction for youngsters. Apparently, the impacts of young pregnancies fluctuate from wellbeing related dangers to enthusiastic torment and uneasiness. The wellbeing dangers are more noteworthy than different dangers, as there are additionally the dangers of contracting explicitly transmitted diseases. In any case, numerous adolescents have concentrated on pregnancies and have disregarded the way that their general regenerative wellbeing may likewise be in danger due early extravagance in sexual related exercises. Despite what might be expected, young pregnancies have discovered manners by which youngsters have looked to get rid of the issue covertly. One of the manners by which the adolescents manage impromptu high school pregnancies is hazardous premature births that the specialists may not know about. Unexpectedly, hazardous premature births are the main executioners inside adolescents with the death rates increasing continuously. In a perfect world, the passing of an age would imply that the instruction framework would endure at long last on the grounds that the disturbing demise rates may upset the learning procedure. In such manner, regenerative wellbeing is the key perspective where the instruction schedule should base its models for educating so as to guarantee the quantity of those that suspend learning lessens by an inconceivable rate. Without this, young pregnancies will always stay a steady obstacle that the general public should experience because of the lack of foresight structures. Basically, the way to limiting the adolescent pregnancies is the use of conceptive wellbeing subjects inside the instruction curricular without society being unassuming in any capacity. Circumstances and end results of young pregnancies Over the years, adolescent pregnancy has become the fundamental driver for the ascent in the quantity of secondary school dropouts. Basically, tutoring structures the fundamental part to a n agreeable future for any little youngster or young lady. For a lady, the measure of tutoring thinks about her expert bearer, her capacity to get hitched, and surprisingly her life principles. In such manner, it is basic for a young lady to decide the chance to which is fitting for her to consider with the goal that her future stays as prosperous as expected. Unexpectedly, youngster childhood is a requesting task henceforth the time spent on this business ought to have been on different issues like tutoring particularly for the youthful grown-ups. Extreme investigates have indicated that early high school pregnancy might be unfavorable to a woman’s life since it raises the odds of her not completing her instruction contrasted with her childless friends. This is to imply that early parenthood brings down their odds of investigating their full

65 preguntas en entrevista residencia por matrimonio

The Disease of Drug Addiction free essay sample

For instance, in his ability as the chief of the National Institute on Drug Abuse, Alan Leshner composed that the explanation we should consider compulsion an infection is that it ‘is attached to changes in cerebrum structure and function’(Leshner,1997). This thinking is reverberated in crafted by a few different creators. Heyman, Heather and Alexander, among others, have tested the ailment status of fixation on essentially experimental grounds (Heyman, 2001; Heather, 1992; Alexander, 1988). Philosophical records of illness, which endeavor to explain the idea, come in numerous shapes and sizes. For instance, Boorse contends for a naturalistic origination of malady wherein an infection must be reflected in lost capacity in an organ (Boorse, 1977). At the opposite finish of the range, Nordenfelt contends for a regulating origination, which characterizes illnesses as conditions which keep us from meeting our ‘vital goals’ (Nordenfelt, 1995). It is as yet an open inquiry whether Boorse’s see, Nordenfelt’s view or some other view gives the best rendering of what we mean when we call something an infection, however the distributed records can bolster the case that adjustments in cerebrum structure and capacity are sufficient to establish a sickness. We will compose a custom article test on The Disease of Drug Addiction or then again any comparative point explicitly for you Don't WasteYour Time Recruit WRITER Just 13.90/page The idea of enslavement as a neurobiological sickness has grabbed hold, on account of the endeavors of both NIDA and the World Health Organization (WHO) that compulsion is an illness (NIDA, 2009 ; WHO, 2004) Substance Dependence or Drug Addiction† The term â€Å"substance dependence† has increased extraordinary cash on account of its utilization in the Diagnostic and Statistical Manual of Mental Disorders (DSM). The DSM, both in its amendment of the third version (DSM-III-R; American Psychiatric Association [APA] 1987) and in its latest release (DSM-IV; APA 1994), maintains a strategic distance from the term compulsion, leaning toward rather to utilize the judgments of substance misuse and reliance, on the whole alluded to as substance use issue. Starting with DSM-III-R, the measures used to analyze substance use issue were applied pretty much similarly to the entirety of the substances that are generally mis-utilized by people. In the DSM, in this manner, people are separated onto three fundamentally unrelated classifications: no substance use issue, misuse just, or reliance. With this methodology, misuse is analyzed just if the individual doesn't meet the standards for reliance. As needs be, an individual gathering the measures for both maltreatment and reliance is determined distinctly to have reliance. The latest content correction of the (DSM-IV-TR; APA 2000, p. 192) recognizes impeded power over substance use as the fundamental element of reliance, which is â€Å"a bunch of intellectual, conduct, and physiological side effects showing that the individual proceeds with utilization of the substance regardless of critical substance-related issues. † The reliance condition, which frames the reason for the symptomatic methodology utilized in DSM-III-R, was first portrayed for liquor by Edwards and Gross (1976); it was later widened to incorporate different medications (Edwards et al. 1981). Be that as it may, as was valid for DSM-III-R (APA 1987), the incorporation of maltreatment as an unmistakable classification in DSM-IV veered off from the simply dimensional methodology (wherein all reliance happens on a continuum, shifting from no reliance indications to serious reliance) taken by Edwards and partners. This dimensional methodology as of late has been upheld by discoveries from a huge, broadly delegate test of in excess of 43,000 individuals. Saha and partners (2006) found that, aside from liquor related lawful issues, all DSM-IV models for liquor misuse and reliance framed a continuum of liquor use issue seriousness. Additionally, just one of four demonstrative standards for liquor misuse (I. e. , perilous use) fell among other measures related with mellow reliance, though the other three maltreatment rules bunched with the most extreme side effects of reliance. These discoveries raise doubt about the differentiation among misuse and reliance and the ID of maltreatment as being milder than reliance. O’Brien and associates (2006) have contended against the utilization of the term substance reliance, requiring a reestablished accentuation on compulsion. Reliance, they brought up, is frequently mistaken for physical reliance (I. e. the adjustments that bring about withdrawal side effects when substance use is stopped), which can happen with helpful utilizations of an assortment of meds. This expressed disarray may make clinicians hesitant to endorse torment meds, for instance, because of a paranoid fear of causing dependence. By underscoring the social parts of habitual substance use, fixatio n catches the incessant, backsliding, and impulsive nature of substance utilize that happens notwithstanding the related negative outcomes. On that premise, these creators encouraged the APA to reestablish the utilization of the term compulsion in the DSM-V, which presently is being developed. A drawback of the term habit, in any case, is that it regularly is utilized disparagingly and can lead professionals to maintain a strategic distance from its utilization because of a paranoid fear of demonizing their patients and harming their relationship with them. Further, the term compulsion has been utilized so broadly and fluidly that, as â€Å"alcoholism,† its significance has been weakened, significantly restricting its worth. The wording used to portray liquor and other medication use issue is of key significance to both the investigation and the clinical consideration of individuals experiencing these conditions (Kranzler, Ting-Kai; 2008). Fixation Is a Disease Drug habit is a habitual conduct that makes a longing to utilize a hazardous substance, regardless of the wellbeing repercussions and some of the time irreversible outcomes. Illicit drug use is an incessant, regularly backsliding cerebrum illness. It is significant not to mistake chronic drug use for tranquilize reliance, sedate reliance doesn't constantly show into illicit drug use. Understanding what occurs in the mind with compulsion is critical to understanding chronic drug use. When a mind is presented to a substance it changes.

A Dialogue of Self and Soul - 11424 Words

TBC02 8/7/2002 04:01 PM Page 46 CHAPTER TWO A Dialogue of Self and Soul: Plain Jane’s Progress a SANDRA M. GILBERT AND SUSAN GUBAR The authors of The Madwoman in the Attic: The Woman Writer and the Nineteenth-century Literary Imagination (1979) are both distinguished feminist critics: Sandra Gilbert is a Professor at the University of California, Davis; and Susan D. Gubar a Distinguished Professor of English and Women’s Studies at Indiana University. They have also collaborated on No Man’s Land: The Place of the Woman Writer in the Twentieth Century, Sex Changes and Letters from the Front with the aim of using feminist criticism to understand the achievements of British and American women in modern times. More recently†¦show more content†¦. . to go all lengths’ (ch. 1). But if Jane was ‘out of’ herself in her struggle against John Reed, her experience in the red-room, probably the most metaphorically vibrant of all her early experiences, forces her deeply into herself. For the red-room, stately, chilly, swathed in rich crimson, with a great white bed and an easy chair ‘like a pale throne’ looming out of the scarlet darkness, perfectly represents her vision of the society in which she is trapped, an uneasy and elï ¬ n dependent. ‘No jail was ever more secure,’ she tells us. And no jail, we soon learn, was ever more terrifying either, because this is the room where Mr Reed, the only ‘father’ Jane has ever had, ‘breathed his last.’ It is, in other words, a kind of patriarchal death chamber, and here 47 TBC02 8/7/2002 04:01 PM Page 48 THE BRONTËS Mrs Reed still keeps ‘divers parchments, her jewel-casket, and a miniature of her dead husband’ in a secret drawer in the wardrobe (ch. 2). Is the room haunted, the child wonders. At least, the narrator implies, it is realistically if not gothically haunting, more so than any chamber in, say, The Mysteries of Udolpho, which established a standard for such apartments. For the spirit of society in which Jane has no clear place sharpens the angles of the furniture, enlarges the shadows, strengthens the locks on the door. And the deathbed of a father who was not really her father emphasizes her isolation and vulnerability. Panicky, she staresShow MoreRelatedThe Ilusory Dialogue In Platos Gorgias1123 Words   |  5 Pagesillusory dialogue that is inaugurated upon Socrates’ analysis of nature and the power Gorgias states are presented in his art, oratory. The first two opening lines of the dialogue forecast the nature of the text, for Callicles and Socrates allude to a Greek saying that is like the Shakespearian saying, â€Å"first at a feast, last at a fray†, when Socrates and his fellow peer, Chaerophon, arrive tardily to the presentation awarded by the famed orator of the time and the namesake of Plato’s dialogue, GorgiasRead MorePlato: Normative Ethical Theory1077 Words   |  5 Pagesof character proper to the just person, in the light of which they decide what actions justice requires of them. In this section we confine ourselves to the character Socrates in Plato s dialogues, and indeed to only certain ones of the dialogues in which a Socrates character plays a role. In those dialogues in which he plays a major role, Socrates varies considerably between two extremes. On the one hand, there is the Socrates who claims to know nothing about virtue and confines himself to askingRead MoreSocrates : The Power Of Knowledge932 Words   |  4 PagesThroughout 470-400 B.C, Greek philosopher Socrates touched many lives of the Athenians and lived to question the knowledge and intelligence of those he met. Socrates took joy in examining the world and self proclaims his own ignorance rather than living under the guise of being an expert. Student of Socrates, philosopher Plato grew in popularity around 400 B.C and strived to uncover the meanings behind ideas such as goodness, reality and beauty. While Socrates became infamous around Athens, and wasRead MorePlato s Philosophy And Philos ophy1070 Words   |  5 PagesPlato, a Greek philosopher, was born around 423 to 428 BC, and died around 347 or 348 BC, at the age of eighty. He was a great philosopher, taught by Socrates. 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It is fair to say that the mind may wonder when one dies what exactly happens to the beloved soul, the giver of life often thought of as the very essence of life does it live on beyond the body, or does it die with it? Does the soul have knowledge of the past if it really does live on? In Plato’s The PhaedoRead MoreSummary : Immortality Of The Soul 1542 Words   |  7 Pagesthe Soul Phaedo, also known to ancient readers as On the Soul, is one of greatest dialogues of Plato’s period. Phaedo centers itself around the death of Socrates. Throug Plato, Socrates lived on generations after his time. Phaedo begins with Socrates addressing his death and stating a true philosopher should look forward to death. He asserts that the soul is immortal and the philosopher spends his life training and detaching itself from the needs of the body. This topic, of â€Å"an immortal soul†, hasRead MorePersonal Identity - Memory Theory vs Body Theory vs Soul Theory1634 Words   |  7 PagesPersonal Identity REFERENCE: Perry, Dialogue on Personal Identity and Immortality. . Thesis . Identity refers to â€Å"a relation that everything has to itself and to no other thing†, and our perception of personal identity is the knowledge that we are ourselves, and who we have been – basically, that I am the same person I was last week, last year, etc. Leibniz’s Law states that if one thing (A) is identical to another (B) at one given point in time, they share the exact same properties, makingRead MoreSocrates Was An Athens A Greek Philosopher1562 Words   |  7 PagesSocrates was an Athens, Greece born man, who is credited as one of the main founders of Western Philosophy. Socrates never wrote any of his thoughts and experiences down, so we just know and analyze Socrates through the dialogues of his greatest apostle, Plato. 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Oral fluid testing device

Sample details Pages: 29 Words: 8588 Downloads: 8 Date added: 2017/06/26 Category Statistics Essay Did you like this example? Abstract CTLS as part of the collaborative xxxxx Trading Ltd for validating One Step Multi-Drug Oral Fluid Drug Testing Device. Also the DOA test device registered with medicines and healthcare products. Validation study of one step multi device was performed in CTLS by two ways. Don’t waste time! Our writers will create an original "Oral fluid testing device | Chemistry Dissertation" essay for you Create order Abbreviations g/L Microgram per litre QA Quality Assurance POCT Point of Care Test MHRA Medicines Healthcare products Regulatory Agency UKNEQAS United Kingdom National External Quality CAP Clinical Pathology Accreditation CTLS Clinical Trials Laboratory AMP Amphetamine BENZO Benzodiazepines COC Cocaine THC -Marijuana MAMP Methamphetamine MOR -Morphine OF Oral Fluid GC Gas Chromatography GC/MS Gas Chromatography Mass Spectrometry LC Liquid Chromatography CE European Conformity DDS Digital data storage 0C Degree centigrade SOP Standard Operating Procedure IVDMD In-Vitro Diagnostic Medical Device Validation method 1 Validation method (1) was conducted from June 2009 through to July 2009. Seven samples were collected from UKNEQAS, which were offered pilot surveys for drugs of abuse in oral fluid and tested using a one step multi device. Three samples were detected by the one step multi device. The one step multi drugs testing device test result was compared to UKNEQAS analyst report. Validation method 2 Validation method (2) started July 2009 and was completed in August 2009, eighteen urine and saliva sample were collected from volunteers and tested for drugs of abuse and tested using one step multi and Dipro Drug lab Multi panel Drug Test devices. During the test, three samples were providing positive results. The test results were compared with each method, and test result would be kept confidential so that clients information would not be disclosed for any other purpose. Chapter: 1 1 Introduction The goal of this project at CTLS was to validate the Drug Abuse Point of Care Testing Kit which will have immense value to the public as well as to the medical research. The validation of testing the kit will certainly in turn become a reliable resource for definitive screening tool in the future. Our aim was to establish clinical cross validation trials necessary to prove drugs of abuse point of care testing kit for human saliva. It could be used as an advanced, accurate and handy clinical laboratory technology to evaluate individuals drug abuse level. Our validation was a team effort to achieve the goal to use human saliva as a non-invasive-bio fluid. The saliva determines the status of a human body. In other words, it is a mirror of the body. The human saliva is easy to collect and can be obtained at low cost in sufficient quantities for analysis. Saliva based tests are more accurate, have a quicker turnaround time and less expensive, particularly as a home drug testing kit. One Step Multi-Drug Oral Fluid Test kit is ideal for qualitative determined level of Amphetamine, Benzodiazepines, Cocaine, Marijuana, Methamphetamine and Morphine in human saliva. This kit provides only preliminary screening of the results within three (3) minutes, and provides the amount of all the drugs present in the human body. If positive result occurs, confirmation must be obtained by more specific chemical method utilizing GC/MS or LC/MS. The drug of abuse of POCT kit will detect, whether any drug is present in oral fluid below the cut-off limit. 1.1 One Step Multi-Drug Oral Fluid Test kit Cut-off Concentrations. Symbol Test Calibrator Point of Care kit Cut-off level(ng/ml) AMP Amphetamine D-Amphetamine 50 BENZO Benzodiazepines Benzodiazepines 30 COC Cocaine Cocaine (parent) 20 THC Marijuana 9-THC(parent) 50 MAMP Methamphetamine D-Methamphetamine 50 MOR Morphine Morphine 40 1.2 The following are the advantages of saliva-based drug of abuse point of care testing kit: Reliable diagnostics and quick turnaround time. Easier than other test methods, which mean no additional equipment and operator training is required. Safer to use, and cheaper than current oral fluid used methods. For example other Oral Fluid methods: GC/MS, ELISA, Point of Care lab tests. Testing kit can be kept at room temperature. Testing provides an accurate and reliable assessment. This test detects most of the common drug abuses. 1.3 Reasons for using the Saliva based Drug Testing Kit: For individual who can check for themselves if there are any drugs presents in their circulation system. Testing can help the employers to determine if any of their current or potential employees abuse any of the banned (illegal) substances. Non-invasive.[19] Sample cannot be adulterated. CE mark. (European Conformity) 1.4 POCT method of DOA test compared with other DOA Test methods Method POCT Oral Fluid ELISA POCT urine GC/MS Sample Oral Fluid Oral Fluid/Urine Urine Oral Fluid, Urine, Blood, Hair Format Lateral Flow ELISA Lateral Flow GC /MS Test time 5min 48-72 hours 5min 3-7days Additional Equipment Required None Incubator ,ELISA reader None Spectrometer Storage RT 2-8 0C RT NA Shelf Life 18 months NA 24 months NA Advantage Accurate reliable Easy to use at any time and any where Non-invasive, No additional equipment required, cheap Accurate reliable High specificity Less expensive Instrument-based testing ,Accurate Disadvantage More expensive than rapid urine testing Expensive, Complex method Sample can be easily adulterated Very expensive 1.5 Oral Fluid Oral fluid is referred to as saliva. The saliva is excreted primarily by 3 glands namely, parotid, sub maxillary and sublingual. It has low protein content and can vary in flow rate from zero to several milliliters depending upon influence from various factors including emotional state and hunger. Spitting or expectoration provide neat saliva but is relatively viscous, and can be difficult to work within the clinical laboratory. It may also be contaminated with food and other particles from the mouth and will therefore require centrifugation. Some of the collection methods used by various companies are those indicated in Table 🙠 1.8.5) and they use some forms of proprietary diluent to mix with collected saliva. The absorbent pad or foam is used to collect saliva comparatively there is no one type of collection device that is better than other when it comes to ease of use. However, there are continuous developments of new products. [6] Plasma electrolytes such as bicarbonate, chloride, potassium and sodium and other plasma elements such as enzyme, immunoglobulin and DNA are present in saliva produced from three major and several minor salivary glands. Plasma electrolytes such as bicarbonate, chloride, potassium and sodium and other plasma elements such as enzyme, immunoglobulin and DNA are present in saliva. Most of drugs can be detected in saliva; including amphetamine, barbiturates, benzodiazepine cannabinoids cocaine methamphetamine ethanol and heroin. [1] However many factors may affect drug transfer into saliva, such as degree of protein binding, pKa, physical size and lipophilicity of drug. 1.6 Advantages of saliva as drug testing matrix Nowadays saliva matrix is more popular for screening of drugs of abuse .The advantages of using saliva matrix are provided below. Saliva collection is easier than other biological fluid. Also direct collection is not embarrassing and significantly reduces an influence of interference enter into sample collection process. Blood collection is a highly invasive method. Testing is more reliable and adaptable for roadside testing. Samples are analysed directly without any further pre-treatment and test results are provided immediately, for example the new brand of Cozard DDS system can provide results within ninety seconds for two major classes of drugs and less than six minutes for six other major classes of drugs. This can help to initiate law enforcement process with sufficient data. An interpretation of drugs in saliva can be used to determine pharmacokinetic limitation. Therefore a low analyte concentration can be easily detected. 1.7 Disadvantages of saliva as drug testing matrix Concentration of drug can be changed by pH during the sample collection. Therefore Saliva and Plasma ratio may be changed. [8] 1.8 Saliva collection methods Production of saliva is influenced by a variety of methods such as stimulating and non-stimulating collection of saliva. [9, 4] 1.8.1 Stimulating method Saliva can be swabbed from mouth using absorbent materials such as sour coated cotton balls. The absorbent material of cotton ball becomes soaked with saliva, which can be removed from the mouth and the saliva is separated by centrifugation or applying pressure to the cotton ball. [3, 4].However, saliva collection method by stimulation is connected with several potential problems, for example the absorbent material can react or absorb some drugs. Therefore actual amount of drug concentration can be varied in saliva. Also some salivary stimulate material (sour flavor) may change the saliva pH level. For example, the level of codeine present in saliva collected after stimulation, which can be decreased by an average of 3.6 times of non-stimulated method of collection. [5].Also non-stimulated saliva contained substantially more drugs than stimulated [8]. 1.8.2 Non-stimulating method Saliva can be collected from mouth into a collection device without using any salivary stimulate material .However, some physical techniques are used to produces saliva ,such as tongue, cheek and lip movements. [8] 1.8.3 Problems with Sample collecting device of saliva Most of sample collection devices contain a form of an inert absorbent pad for saliva collection [9]. However, different amount of saliva is absorbed during the sample collection process by different devices. Collection of sample is not assured by most of sampling devices. Therefore many of devices have a colour changing indicator. An absorbent pad made from different materials. This material must be chosen carefully because some materials may absorb some of the drug. This can lead to errors. Different buffers /preservatives which used in different devices may be incompatible with hyphenated MS techniques for a confirmation of drug abuse levels [4]. Some of the most common problems: ion suppression in LC-tandem MS long term contamination of columns and ion source in GC 1.8.4 Advantages of sample collecting devices The point of care saliva collector uses an optimal method for obtaining full saliva. Saliva is easily obtained by chewing on a roll-shaped saliva collector and Very Cheap. The chewable kit are coated with citric acid .This can be stimulated more saliva. 1.8.5 The list of saliva sample collection devices and characteristics Collection Device Made in Method of collection Control of, saliva volume Buffer /diluents Stimulated collection Cozartoral swab. UK Absorbent swab Yes Yes No Intercept Device. USA Salt impregnated pad No Yes Yes Drug Wipe. Germany Absorbent pad Yes No No Greiner Bio-One. Austria Saliva extraction solution Yes Yes No Saliva Twist device Drug Test. UK Sponge swab No Yes No Oralab 6. USA Absorbent pad Yes No No Oraline. USA Collection cup Yes No No Oral Stat. USA Sponge swab No Yes No Oratect. USA Absorbent pad Yes No No VerOFy.USA Absorbent pad Yes No No Saliva Screen. Germany Absorbent pad Yes Yes Yes, acidic available mechanical Smart clip multidrug. Germany Absorbent pad No Yes Yes, mechanical Quantisal. USA Absorbent pad Yes Yes No Salivette. Germany Cotton wool/polyester swab No No Yes, acidic available mechanical Source: young scientist journal June 2009 and the kit manufactures web sites 1.9 Literature Reviews (1) Biermann et al., 2004 did research on On-site testing of illicit drugs using Toxiquick. The experiment was carried out for 18 months. During the periods 751 individuals were tested at road site with immunochemical test device Toxiquick for drug abuse such as amphetamine, MDMA, MDE, cannabis, cocaine, heroin and morphine. 302 individuals oral and blood samples were taken during the periods. This was tested with immunochemical and non immunochemical method (GC-MS) and the test results were compared. In general 75% Toxiquick results were agreed with GC-MS results. However, cannabinoids test were shown to have negative values. This can be caused by metabolise. (2)Vanna De Rose et.at(2004) wrote a review report on drug testing in the work place, and the summary of their review report is given below directly quoted from their work. Drug testing in the work place: There are various methods of approaches to identify drugs of abuse for staff . For example urine, blood, oral fluid, hair and sweat can be used to test. Four fundamental reasons concern the author, for drug testing at work place; the cost of drug testing at work place, how reliable are the results? Cut off levels and strength of drug testing device. Safety: drugs of abuse can increase safety risk to own self and co-workers at work place, for example, where employees are working at higher sensitivity working area such as air traffic controllers, Pilots and Ambulance drivers etc. Organizational efficiency: drugs of abuse can increase low productivity, high staff turnover and absenteeism. Therefore drugs testing can increase the productivity and reduce the cost of new employees and training them. Reputational risk: When an employee takes an illegal drug, the reputation of the organization can be damaged. Employee welfare: Drug test can improve employee health and would help to identifying staff that need to be sued for drug intake. The cost of drug testing at work place can be influenced by three major factors. Higher financial costs are involved for drug test. Drug testing can be damaged relationship between staff. Employment of staff and human resource service are a significantly higher cost. Testing device can detect drugs other than illicit drugs. For example over-the-counter painkillers can be detected and have produced a false positive result because of the opiate. This problem can cause discrimination against a person who is accused of false impairment. The problem can be resolved by changing of identification cut off limits for specific level of illicit drugs. Most of drug testing device can indicate only drug metabolite activity in body but not the level of intoxication. (3)E.J. Cone. et.al (2007) in their paper on Interpretation of oral fluid test for drugs of abuse The article reviewed several aspects of oral fluid testing, detection times, application of oral fluid testing and interpretation of test result .They also reported the influence of chemical, physiological and pharmacological factors that are involved in oral fluid testing result. (4)Prof Olaf H Drummer. Clinical Biochemist Reviews (2006, August 27(3): 147-159) In The Clinical Biochemist Journal the application of method of testing for drugs in saliva such as LC-MS, HPLC with MS are compared with road site drug testing kit. The Advantages of testing kit were described, in the Journal, to be a level of accuracy and efficiency. (5)Kato et al .J Anal Toxicology 1993 Oct: 17(6):338-41 Cocaine and metabolite excretion in saliva under stimulated and non-stimulated conditions .The collections of saliva sample were carried out by stimulated or non-stimulated condition from six healthy volunteers who were given cocaine during the test session. The presence of cocaine level and metabolites were analysed by GC-MS .They reported that the non-stimulated saliva contained more cocaine than stimulated. This can be caused by levels of pH present in saliva. (6) Dr.Graham Facile. Evaluation of the Drager Drug Test 5000Test System. (2008): Drager Drug Test 5000 test system has been used for detecting drugs in human-saliva /oral fluid. During the period of testing, 503 individual tests were done .The test results obtained were within minutes utilizing Drager Drug Test Test System .Also this device can detect the trace level of drugs present in saliva, for example, Cocaine (20ng/ml), Opiates (20ng/ml), Benzodiazepine (15ng/ml), Delta -9-THC (25 ng/ml) and Amphetamine (50 ng/ml).The results were compared with drugs of abuse point of care kit cut- off values. The Drager Drug Test 5000 Test System was found as more powerful immunochemical based diagnostic medical device. (7) N.Fucci et.al SPME-GC analysis of THC in saliva sample collected with EPITOPE device. (2001).The article mentioned several factors about oral fluid analysed with different techniques, method of collection device and oral fluid reflection for drug concentration. They reported that the drug concentration in saliva is directly reflected to plasma concentration in body. They detected a higher level of cannabinoid in saliva. So, this could be due to the fact that cannabinoid smoke is directly saturated in the oral cavity. They used an EPITOPE device, which has an absorbent pad or foam used to collect saliva. (8)O.R.Idowu and B.Caddy A Review of the use of Saliva in the forensic detection of drugs and other chemicals (2008).In their review they reported the saliva sample to be useful for drug detection in the forensic analysis. Concentration of drug level in Plasma can be reflected in saliva. (9)Schramm.W et.al Drugs of abuse in saliva Journal of Analytical Toxicology (1992) This article reviewed various aspects of drugs of abuse in saliva. Analytical methods were used for the detection of drugs, including gas chromatography with mass spectrometry, thin layer chromatography and immunoassay. The study included that the level of concentration of cocaine and phencyclidine is correlated with saliva and plasma. They also found that the drug concentration of tetrahydrocannabinol (marijunana) can be reflected in saliva and blood. (10)Dennis .J.Crouch et.alAn Evaluation of innovation Sweat Based Drug Testing Techniques for use in Criminal Justice Drug Testing April 2002 The article discussed the acceptable drug/metabolites confirmations techniques such as GCMS / LCMS and LC/MS/MS. However, the researcher pointed out that the cannabinoid (THC) drug analysed with LC/MS/MS, can cause some interference, such as, electron spray ionization due to lack of a functional group ionized made electron spray. (11) Creative Research Systems. https://www.surveysystem.com/sscalc.htm. National Statistical Service. https:// www.abs.gov.au These articles provide the relationship between the sample numbers, confidence level and the confidence interval. Chapter: 2 2 Validation Method This study of saliva matrix based on drugs of abuse point care testing kit [DAPOCTK] validation was performed in CTLS using two methods. 2.1 Validation Method: 1 A validation of Drug Abuse Testing Point of Care Kit [DAPOCTK] was performed by participating in accordance with United Kingdom National External Quality Assessment Scheme [UKNEQAS] for oral fluid Drugs of Abuse. 2.1.1 Role of United Kingdom National External Quality Assessment Scheme [UKNEQAS] The UKNEQAS can offers a number of proficiency testing schemes for monitoring drug assays in serum, pilot surveys for drugs of abuse in saliva toxicological investigations and drugs of abuse testing in urine. Also a quality assurance service with strong educational knowledge is provided. 2.1.2 How does the Pilot Surveys Scheme work? Known coded samples will be distributed quarterly and drug analysis will be performed for 4 weeks. The analytical performance will be informed to participated laboratories. 2.2 Validation Method: 2 The validation purpose of this study, urine and saliva specimens were required from volunteers and tested for drugs of abuse with one step multi and Dipro Drug lab Multi panel Drug Test devices. 2.2.1 Outcome of Comparison of two POCT aid Validation: Both CE marked POC testing kit are ideal for qualitative determined level of drugs of abuse in human bodies using human biological specimens. Saliva matrix based on POC testing kit results correlate with urine matrix POCT kit result. The Saliva matrix based on POC testing kit will be validated by urine matrix POCT kit result. 2.2.2 The voluntary participation with CTLS for validation of POCT kit purposes: The identities of participants will not be disclosed or divulged to the scientist / other staff members of CTLS who receive sample of oral-fluid /saliva. The purpose of Drug of Abuse Information and Consent Form [Appendix II] is to provide information pertaining to voluntary participants of drugs-abuse and CTLS for research purposes as to the nature of the role and the legal and ethical standards to be met. 2.2.3 Scope of the Legalities: To protect the confidentiality of the identities of the voluntary participants any information about the participants will not be revealed. Steps will be taken to protect the confidentiality of voluntary participants data including code numbers, database, and storage of research data in CTLS. Chapter: 4 4 Registered a point of care testing kit with Medicines and Healthcare products Regulatory Agency. 4.1 Medicines and Healthcare products Regulatory Agency. The Medicines and Healthcare products Regulatory Agency (MHRA) is United Kingdom government agency of the Department of Health. 4.2 Role of MHRA MHRA is responsible for making sure that medicine and medical devices work with safety regulation in UK 2002. Medicines and medical devices information are provided so that they are publicly available. MHRA is monitoring medicines and medical devices in market and encourage the public to inform them of any problem with a medicines and medical device. If there are any problems with medicines and medical devices, then they are removed from market, so that they can be investigated and the necessary action needed can be taken against manufacturer. 4.3 In Vitro Diagnostic Medical Devices Register with the MHRA CE marking declaration certification [Appendix III] Registration Form RG3 Registration fees The required information for registration process and other pertinent information can be found in the MHRA Web site. https://www.mhra.gov.uk/Howweregulate/Devices/Registrationofmedicaldevices/CON009810 Chapter: 5 5 Method Validation Plan: Title: Validation of Drugs of Abuse Point of Care Testing kit Objective: To validate Drug Abuse Testing Point of Care Kit [DAPOCTK] by participation of the United Kingdom National External Quality Assessment Scheme for oral fluid drugs of abuse. The POCT kit will be validated using UKNEQAS oral fluid samples in Clinical Trials Laboratory Services Ltd. The POCT kit will be validated by comparison with Volunteer urine samples screening DOA test in Clinical Trials Laboratory Services Ltd. 5.1 Validation of Analytical Method 5.1.1 Reference Compound, Apparatus and POCT devices 5.1.2 Reference Compound of Oral Fluid UKNEQAS Reference Item will be analysed for validation. Identity: Oral Fluid Provide by: United Kingdom National External Quality Assessment Schemes Batch No: OF/1008/October 08, OF/0609/June 09, Oral Fluid: (OF) 5.1.3 Apparatus Micro Pasteur pipette (10l) 5.1.4 Different matrices of POCT devices 5.1.4.1 Saliva matrix based on a point of care test kit. Trade name of kit: XXXX Oral Fluid Test kit. Manufacture: XXXXX.Ltd in China Batch No: W650690510. Expiry date: 05/2011. Drug tests for: COC/Amp/mAmp/THC/MOP/BZO. CE marked by Qarad European Regulatory Service in Belgium. XXX Oral Fluid Test kit contained: Saliva Test Panel, Saliva Collection tube, Saliva Sponge Collector. 5.1.4.2 Urine matrix based on a point of care test kit. Trade name of kit: XXXXXX Multi panel Drug Test Manufacture: XXXXX in Austria. Batch No: DOA8110062. Expiry date: 01/2010. Drug tests for: COC/Amp/mAmp/THC/MTD/MOP/PCP/BAR/BZO/TCA XXXX Multi panel Drug Test kit contained: Urine Test Panel 5.2 Number of sample determination: For determinations of sample size the following factors should be considered. How accurate the result needs to be? The level of confidence limit of the test results. Available budgets for sampling and analysis. The number of samples needed for validation can be determined by two calculation methods. 5.2.1 Calculation method: 1 S = (z/e) 2 S -The sample size, Z -The degree of confidence (95%, 1.96) 5.2.2 Calculation method: 2 S = [Z2 x (p) x (1-p)] / C2 S -The sample size, Z -The degree of confidence (95%, 1.96), C Accepted error value as proportion, P An estimate of the proportion of numbers of samples falling into the group of representing the population. A larger number of samples truly reflect the population size. However, the experiment will be conducted with a minimum number of samples. Therefore the confidence interval percentage will be chosen as +/-19.6 and number of samples found as 25 at confidence level 95% [2, 10].In this validation of POCT kit experiment, the calculation method (1) will be used for determination of number of samples. 5.3 Experimental Pathways: This validation of this experiment can be carried out by two different experimental pathways. However, the total number of samples will be maintained. (n= 25) 5.3.1 Experiment Pathway: 1 Saliva based on drugs of abuse POCT kit validation process can be carried out by UKNEQAS oral fluid samples .Oral Fluid will be directly introduced in to POC testing panel, where a sample introduction place [Appendix 1 (4)]. This validation process can be taken over a period of one year. 5.3.2 Experiment Pathway: 2 Saliva based on drugs of abuse POCT kit validation process can be carried out by urine based on drugs of abuse POCT kit. This validation process will be completed in a short time frame. The short period of time will depend on availability of sample. A portion of the saliva and urine sample will be introduced into appropriate matrix of POC testing panel, where a sample introduction place. [Appendix 1 (4)] 5.4 Description of the Validation Procedure: 5.4.1 Sample collection methods: Saliva or oral fluid and urine samples will be collected from healthy volunteers or from patients taking known medication. 5.4.1.1 Saliva sample: Saliva directly collected from the mouth using saliva sponge collector. Then the saliva saturated sponge will be squashed in to saliva collector device. [Appendix: I (2, 3)] 5.4.1.2 Urine sample: Urine sample directly collected from volunteers. 5.4.1.3 UKNEQAS sample: UKNEQAS Oral fluid samples will be collected from volunteers or patients taking known medication. 5.4.2 Sample pre-treatment: UKNEQAS Oral fluid samples will be diluted with oral fluid free of all abused drug groups. The UKNEQAS oral fluid samples concentration will be adjusted for investigation of laboratory performance levels. The UKNEQAS oral fluid samples will be preserved by heating at 60 oC for 90 minutes and addition of bronidox 0.05%, gentamicin 0.1g/L and penicillin 0.1 g/L.[UKNEQAS Oral Fluid pilot surveys for drugs of abuse in saliva guide line 2009] 5.4.3 Handling procedure for POCT device and sample: All POCT devices will be operated in accordance with manufactures instruction. After use POCT device will be disposed in accordance with applicable law. All POCT devices will not used beyond their expiration dates. All biological specimen samples should be considered as potentially hazardous. All used POCT device and biological specimen can transfer infections. 5.4.4 Composition of Validation Run: Each biological specimen will be performed twice for validation run. A test result is indicated by colour or faint band in the Test Valid Window of POCT kit. 5.4.5 Sensitivity: The drug of abuse of POCT kit will detect if any drug is present in oral fluid above the cut-off limit. 5.4.6 One Step Multi-Drug Oral Fluid Test kit Cutoff Concentrations Symbol Name of Drug Calibrator POCT kit Cut-off level(ng/ml) AMP Amphetamine D-Amphetamine 50 BENZO Benzodiazepines Benzodiazepines 30 COC Cocaine Cocaine (parent) 20 THC Marijuana 9-THC(parent) 50 MAMP Methamphetamine D-Methamphetamine 50 MOR Morphine Morphine 40 5.4.6 Specificity: A stimulating saliva collection method is connected with several potential problems, for example, the absorbent material can react or absorb some drugs. Therefore, the actual amount of drug concentration can vary in saliva. A salivary stimulate material (sour flavor) may change the saliva pH level. For example, the level of codeine present in saliva collected after stimulation can be decreased by an average of 3.6 times of non-stimulated method of collection. [4].Therefore, non-stimulated saliva contains a substantially higher amount of drugs than stimulated saliva. [8]Testing devices can detect other than illicit drugs. For example, over-the-counter painkillers can be detected and have produced a false positive result because of the opiate [15] A higher level of cannabinoid is detected in the saliva. This could be due to cannabinoid smoke being directly saturated in the oral cavity. [9] The level of concentration of cocaine and phencyclidine is correlated with saliva and plasma. Also it was found that tetrahydrocannabinol (marijuana) drug concentration can be reflected in saliva and blood. [13] 5.4.7 Limitation of Procedure: This One Step Multi-Drug Oral Fluid testing kit is designed for detection of drugs in human saliva only. A positive result will be visibly obtained on test window in the kit. Therefore only the presence of a drug is found in testing sample. However, the test result can indicate a false result due to technical or procedural error. This testing kit cannot be measured or indicate the intoxication level in the body and distinguish between drug of abuse and medicines. 5.4.8 POCT device and Sample Storage: A drug of abuse testing kit will be kept at room temperature (15-30oC) in the original foil pouch. Saliva or oral fluid will be stored at 4oC in refrigerator for up to 2 days or frozen at -20 oC until use. 5.4.9 Interpret Test Validity and Test Result: Test validity and result will be interpreted within 5 minutes after initiating the test and result confirmation will be reported within 10 minutes. 5.4.9.1 Interpret Test Validity: The test validity is indicated by formation of a colour band in the POCT device Test Valid window. However, the development of colour bands may require up to 15 minutes in the Test Valid Window. This can be caused by the high viscosity and variability of saliva sample. 5.4.9.2 Interpretation of Test Result: The test result will be interpreted as either negative or positive immediately. However, the random error of visual test result interpretation will be determined by three analysts. If three individuals visual test results will be found as significantly different, confirmation must be obtained by more specific chemical method using GC/MS or LC/MS. 5.4.9.2.1 Positive Test Result: There is a positive result when the coloured band cannot be observed in test valid window next to the specified drug name. Therefore specified drugs can be found in saliva above the cut off limit. However, one coloured band observed in the control valid window, has to be present for the results to be valid. 5.4.9.2.2 Negative Test Result: Two coloured band can be observed. One colour band can be observed in test valid window next to the specified drug name and the other colour band can be observed in control valid window. 5.4.9.2.3 Invalid Test Result: Coloured band cannot be observed in control valid window. Chapter: 6 6 Result: 6.1 Table R: 1 Results for volunteer saliva and urine samples screened with POCT. Sample Saliva Sample Screening with POCT Result Urine Sample Screening with POCT Result ID AMP BENZO COC THC mAMP MOP AMP BENZO COC THC mAMP MOP 0330661 N N N N N N N N N N N N 0330662 N N N N N P N N N N N P 0330644 N N N N N P N N N N N P 0330670 N N N N N N N N N N N N 0330675 N N N N N N N N N N N N 0330691 N N N N N N N N N N N N 0330692 N N N N N N N N N N N N 0330701 N N N N N N N N N N N N 0330702 N N N N N N N N N N N N Note: AMP: Amphetamine, BENZO: Benzodiazepine, COC: Cocaine, THC: Marijuana, mAmp: methamphetamine, MOP: Morphine, P = positive, N = negative, U=uncertain or numeric result. OF: oral fluid 6.2 Table R: 2 Results for UKNEQAS oral fluid (Oct 2008) sample screened in CTLS with One Step Multi-drug POCT and UKNEQAS Analyst Report. Sample Oral fluid sample Screening in CLTS with POCT UKNEQAS analyst result report ID AMP BENZO COC THC mAMP MOP AMP BENZO COC THC mAMP MOP OF11008 P N P N N N P N N N N N OF21008 N N N N N N N N N N N N OF3 1008 N N N N N N N N N N N N OF4 1008 N N P N N N N N N N N N Note: AMP: Amphetamine, BENZO: Benzodiazepine, COC: Cocaine, THC: Marijuana, mAmp: methamphetamine, MOP: Morphine, P = positive, N = negative, U=uncertain or numeric result. OF: oral fluid 6.3 Table R: 3Results for UKNEQAS oral fluid (June 2009) sample screened in CTLS with One Step Multi-drug POCT and UKNEQAS Analyst Report. Sample Sample Screening Result in CLTS UKNEQAS analyst result ID AMP BENZO COC THC mAMP MOP AMP BENZO COC THC mAMP MOP OF10609 N N N N N N N N N P N N OF20609 N N N N N N N P N N N N OF30609 N N N N P N P N N N N N Note: AMP: Amphetamine, BENZO: Benzodiazepine, COC: Cocaine, THC: Marijuana, mAmp: methamphetamine, MOP: Morphine, P = positive, N = negative, U=uncertain or numeric result. OF: oral fluid Chapter: 7 7 Discussion During POCT validation process, there were a number of difficulties encountered in collection samples to obtain valid data. One of the problems identified was poor sampling transportation. Therefore cannabinoid group and Delta-9-THC drugs initial concentration can be decomposed or metabolized by directly contact with plastic vials as such long hours. Second problem was that when using a One Step Multi drug POCT kit detect over-the-counter painkillers, false positive result was provided due to the opiate. Volunteers saliva and urine sample were tested with saliva matrix POCT kit and urine matrix POCT kit for drugs of abuse. The results were shown table R1. Two out of six (6) sample results had morphine in them 0330662 and 0330644.One of the two test results was confirmed by volunteers medical history. The other result was not confirmed. This is because the volunteer did not disclose any information. Also the saliva and urine POCT kit did not detect for Amphetamine, Benzodiazepine, Cocaine, Marijuana and Methamphetamine in saliva. This could have been due to the presence of lower level of drug concentration in the oral fluid and urine specimens, which was below the device designated cut-off limit. UKNEQAS sample batch (2008) of oral fluid samples were tested with One Step Multi drug POCT kit in CTLS lab for specific six drugs namely, Amphetamine, Benzodiazepines, Cocaine, Marijuana, Methamphetamine and Morphine. The results were shown table R2. Amphetamine and Cocaine drugs were correctly identified by employing an immunoassay based on One Step Multi drug POCT kit in oral fluid OF1 1008, OF4 1008 respectively. In the following UKNEQAS (Nov 2008) report, eighteen out of the twenty-two laboratories tested had positive results of amphetamine in oral fluid OF11008 was reported. Ten laboratories correctly identified the amphetamine using hyphenated mass spectrometry techniques. Two laboratories results showed the detection of amphetamine, using ELISA method and the results were confirmed by GCMS techniques. Additionally, a UKNEQAS analyst was informed, 52.2g/L of amphetamine was spiked in oral fluid OF1 1008.The One Step Multi drug POCT kit cut-off value for amphetamine was reported as 50 g/L by manufacturer. Also Cocaine was detected in oral fluid OF11008 and OF4 1008 respectively by one step multi drug POCT in CTLS. In addition, eight out of twenty-two laboratories results were reported to contain cocaine in oral fluid OF4 100 8. However, the UKNEQAS analyst report (Nov 2008) mentioned no correct report has been entered for the cocaine metabolite group represented by the presence of benzoylecgonine at a concentration above that for the single analyt (8g/L) but not that for the group (20g/L). UKNEQAS sample batch (2009) oral fluid samples were tested with One Step Multi drug POCT kit in CTLS lab for six particular drugs namely, Amphetamine, Benzodiazepines, Cocaine, Marijuana, Methamphetamine and Morphine. The results were shown table R2. The One Step Multi-Drug test device gave more false negative results than valid results for cannabinoid group, benzodiazepine group and amphetamine group, from UKNEQAS oral sample batch June 2009. There were possible reasons: 7.1 Cannabinoid group: Initial, the oral sample was spiked with 19.8 ng/ml of delta-9 tetrahydrocannabinol. The sample arrived two days later by post. Therefore cannabinoid group and Delta-9-THC drugs initial concentration can be decomposed or metabolized by direct contact with plastic vials during such a long period of time. Also One Step Multi-Drug test device cut-off limit for delta-9 tetrahydrocannabinol was reported as 20ng/ml. 7.2 Benzodiazepine group: Initial, the oral sample was spiked with 11.5ng/ml of Benzodiazepine group. However, One Step Multi-Drug test device cut-off limit for Benzodiazepine group was reported as 30ng/ml. 7.3 Amphetamine group: Initial, the oral sample was spiked with 52.3ng/ml of MDMA (3, 4-methyl enedioxymethylafetamineand) 17.6 ng /ml of MDA. (Methyl ene dioxymethylafetamine) However, One Step Multi-Drug test device was made for amphetamine immunoassay only. Chapter: 8 8 Conclusion The One Step Multi-Drug test device was sensitive enough to detect the THC and term of amphetamine group, in UKNEQAS external quality assessment scheme for drugs in oral fluid (June 2009) as reported in results. The outcomes of the Validation of POCT device raise several interesting analytical problem that could resolve in further code known sample analysis. Appendix II Consent Form Drugs of Abuse Information and Consent Form Information and Consent Form for volunteers to donate Saliva, and Urine samples to Clinical Trials Laboratory Services for research purposes.Fresh human Saliva and Urine are required for research purposes by Clinical Trials Laboratory Services (CTLS) clients. These clients use the Saliva and Urine to help with basic research into various drugs of abuse. Please note that genetic research (DNA genotyping studies) will NOT be performed using these bio samples. Any remaining unused saliva and urine product will be discarded once a specific piece of work has been completed. However, in a few instances processed samples may be stored for later subsequent research. You are being asked to give consent for your saliva and urine to be used by scientists for research purposes. To protect your privacy under the Data Protection Act your sample will be labeled (or coded) only with a subject number, not your name or any other personal identifier. Some information (e.g. donor gender and age) may also be supplied with your sample in a similarly coded fashion to the scientists. Only CTLSs authorized Clinical Study Unit staff will have access to the link between your subject number and your name. In this way, your identity will not be divulged to the scientists who receive your saliva and urine sample. Your participation is voluntary; you are free to withdraw consent and leave the Volunteer Panel at any time without explanation. After each donation you will be reimbursed for your inconvenience and time. The results of any research might be valuable for commercial and/or intellectual property purposes (patenting for example). If you decide to become a volunteer, you are giving your sample to CTLSs clients who will retain sole ownership of any such research results and of any use or development of the research records (including your sample) consistent with this consent. You will not receive any financial benefit that might come from the research results. Consent My signature below indicates that: I have read and understand this form and all the information given to me. I have been given the opportunity to discuss the donation and ask questions. I am satisfied with the answers. I wish to be part of the CTLS Saliva and Urine Donation Volunteer Panel. I understand that my Saliva and Urine will be used for non-genetic research purposes. I understand that I will not be able to receive the results of any research. I will have no ownership of the research results or the research records. I agree that CTLSs clients may apply for and use patents relating to the research results, records and developments. I do give consent to take _______________ ml of saliva. I do give consent to take _______________ ml of urine. Volunteers name (Please print) Signature of Volunteer Date ________________________ _____________________ _________ Volunteer is fit for blood, urine and saliva donation. Screening Staff: ___________________Signature: ________________ Witness Name: ____________________Witness Signature: _____________________ Contact no: _________________E-mail ID: ___________________________ A.IV.3Saliva and URINE SAMPLE test result CLINICAL TRIALS LABORATORY SERVICES PRE SCREENING STUDY CHECK LIST Drugs of Abuse Result sheet: Study No: 339 Date: 07/07/2009 Lab Ref No: 0330661 Name of Drug Result : Saliva Result: Urine AMP N N BENZO N N COC N N THC N N MAMP N N MOR P P Date: 07/07/2009 Analyst: S.Mayuran Note: AMP- Amphetamine, BENZO-Benzodiazepines, COC-Cocaine, THC-Marijuana, m-Amp-Methamphetamine, MOR-Morphine .P=Positive, N= Negative. CLINICAL TRIALS LABORATORY SERVICES PRE SCREENING STUDY CHECK LIST Drugs of Abuse Result sheet: Study No: 339 Date: 08/07/2009 Lab Ref No: 0330662 Name of Drug Result : Saliva Result: Urine AMP N N BENZO N N COC N N THC N N MAMP N N MOR N N Date: 08/07/2009 Analyst: S.Mayuran Note: AMP- Amphetamine, BENZO-Benzodiazepines, COC-Cocaine, THC-Marijuana, m-Amp-Methamphetamine, MOR-Morphine .P=Positive, N= Negative. CLINICAL TRIALS LABORATORY SERVICES PRE SCREENING STUDY CHECK LIST Drugs of Abuse Result sheet: Study No: 339 Date: 08/07/2009 Lab Ref No: 0330664 Name of Drug Result : Saliva Result: Urine AMP N N BENZO N N COC N N THC N N MAMP N N MOR P P Date: 08/07/2009 Analyst: S.Mayuran Note: AMP- Amphetamine, BENZO-Benzodiazepines, COC-Cocaine, THC-Marijuana, m-Amp-Methamphetamine, MOR-Morphine. P=Positive, N= Negative. CLINICAL TRIALS LABORATORY SERVICES PRE SCREENING STUDY CHECK LIST Drugs of Abuse Result sheet: Study No: 339 Date: 13/07/2009 Lab Ref No: 0330670 Name of Drug Result : Saliva Result: Urine AMP N N BENZO N N COC N N THC N N MAMP N N MOR N N Date: 13/07/2009 Analyst: S.Mayuran Note: AMP- Amphetamine, BENZO-Benzodiazepines, COC-Cocaine, THC-Marijuana, m-Amp-Methamphetamine, MOR-Morphine, P=Positive, N= Negative. CLINICAL TRIALS LABORATORY SERVICES PRE SCREENING STUDY CHECK LIST Drugs of Abuse Result sheet: Study No: 339 Date: 21/07/2009 Lab Ref No: 0330675 Name of Drug Result : Saliva Result: Urine AMP N N BENZO N N COC N N THC N N MAMP N N MOR N N Date: 21/07/2009 Analyst: S.Mayuran Note: AMP- Amphetamine, BENZO-Benzodiazepines, COC-Cocaine, THC-Marijuana, m-Amp-Methamphetamine, MOR-Morphine, P=Positive, N= Negative. CLINICAL TRIALS LABORATORY SERVICES PRE SCREENING STUDY CHECK LIST Drugs of Abuse Result sheet: Study No: 339 Date: 28/07/2009 Lab Ref No: 0330691 Name of Drug Result : Saliva Result: Urine AMP N N BENZO N N COC N N THC N N MAMP N N MOR N N Date: 28/07/2009 Analyst: S.Mayuran Note: AMP- Amphetamine, BENZO-Benzodiazepines, COC-Cocaine, THC-Marijuana, m-Amp-Methamphetamine, MOR-Morphine, P=Positive, N= Negative. CLINICAL TRIALS LABORATORY SERVICES PRE SCREENING STUDY CHECK LIST Drugs of Abuse Result sheet: Study No: 339 Date: 29/07/2009 Lab Ref No: 0330692 Name of Drug Result : Saliva Result: Urine AMP N N BENZO N N COC N N THC N N MAMP N N MOR N N Date: 29/07/2009 Analyst: S.Mayuran Note: AMP- Amphetamine, BENZO-Benzodiazepines, COC-Cocaine, THC-Marijuana, m-Amp-Methamphetamine, MOR-Morphine, P=Positive, N= Negative. CLINICAL TRIALS LABORATORY SERVICES PRE SCREENING STUDY CHECK LIST Drugs of Abuse Result sheet: Study No: 339 Date: 05/08/2009 Lab Ref No: 0330701 Name of Drug Result : Saliva Result: Urine AMP N N BENZO N N COC N N THC N N MAMP N N MOR N N Date: 21/07/2009 Analyst: S.Mayuran Note: AMP- Amphetamine, BENZO-Benzodiazepines, COC-Cocaine, THC-Marijuana, m-Amp-Methamphetamine, MOR-Morphine, P=Positive, N= Negative. CLINICAL TRIALS LABORATORY SERVICES PRE SCREENING STUDY CHECK LIST Drugs of Abuse Result sheet: Study No: 339 Date: 05/08/2009 Lab Ref No: 0330702 Name of Drug Result : Saliva Result: Urine AMP N N BENZO N N COC N N THC N N MAMP N N MOR N N Date: 28/07/2009 Analyst: S.Mayuran Note: AMP- Amphetamine, BENZO-Benzodiazepines, COC-Cocaine, THC-Marijuana, m-Amp-Methamphetamine, MOR-Morphine, P=Positive, N= Negative. References: Biermann. T,Schwarze B,Zedler.B and Betz.POn-site testing of illicit drugs Journal of Forensic Science International 143 (2004) 21-25. Creative Research Systems.411B Street Suite2 Petaluma CA 94952 Tel: (707)765-1001 https://www.surveysystem.com/sscalc.htm online. 30th June 2009 Dennis .J. Crouch, Dr. Royer F Cook, Dr James V.Trudea,David C.Dove An Evaluation of Innovation Sweat Based Drug Testing Techniques for use in Criminal Justice Drug Testing U.S. Department of Commerce, Office of Law Enforcement Standard.2002,206825.https://www.eeel.nist.gov. Accessed 30th June 2009 Dennis J.Crouch et.al Oral fluid collection: The neglected variable in oral fluid testing. Journal of Forensic Science International 150 (2005) 165-173. Dr.Graham Facile Cope, Honorary Senior Research Fellow. Evaluation of the Drager Drug Test 5000Test System. University of Birmingham 2008 November. Edward J. Cone and Marilyn A Huestis Interpretation of Oral Fluid Tests for Drugs of Abuse. Ann NY Acad Sci.2007 March 1098, 51-103. Jayne E. Thatcher. ROSITA II Project: Evaluation of On-Site Saliva Drug Testing Devices in Washington State.2007 Kato K,Hillsgrove M,Weinhold L,Gorelick DA,Darwin WD,Cone E J Cocaine and metabolite excretion in Saliva under Stimulated and non-Stimulated conditionsJ Anal Toxicology 1993 Oct: 17(6):338-41 N.Fucci,N.De Giovanni,M.Chiarotti,S.Scarlata.SPME-GC analysis of THC in saliva sample collected with EPITOPE device. Journal of Forensic Science International 2001 Volume 119, Issue 3,318-321. National Statistical Service, Australian Bureau of Statistical, PO Box 10 Belconnen ACT 2616, Austrialia. https:// www.abs.gov.au accessed 03rd July2009 O.R.Idowu and B.Caddy A Review of the use of Saliva in the forensic detection of drugs and other chemicals (1982) Journal of Forensic Science Society Volume 22, Issue 2,123-135. https:// www.icadts2007.org/print/80rosita_salivascreen.pdf Accessed 03rd July 2009 Prof Olaf H Drummer. Drug Testing in Oral Fluid Clinical Biochemist Reviews (2006) August 27(3): 147-159. Schramm.W.Smith RH, Craig PADrugs of abuse in saliva Journal of Analytical Toxicology (1992)16:1-9.39 UKNEQAS Oral Fluid pilot surveys for drugs of abuse in saliva guide line 2009]. http:/ www.heathcontrol.com Accessed 03rd August 2009. Vanna De Rosas,Marcus Roberts,Ruth Evans,Yolande BurginDrug testing in the work place the report of the independent inquiry into Drug Testing at work. the Joseph Rowntree Foundation. 2004 online pdf ISBN 185935212X, www.jrf.org.uk. Accessed 03rd August 2009 Vincent Cirimele, Marion Villain, and Patrick Mura Marc.Bernard Pascal Kintz Oral fluid testing for cannabis: On-site Oraline IV s.a.t device versus GC/MS.Forensic Science International 16, 2006, 180-184. Walsh J.M, Flegel R., Crouch D.J, Cangianeli L, Baudys J. An Evaluation of Rapid Point-of-collection Oral Fluid Drug-Testing Devices Journal of analytical Toxicology, volume 27,No 7, October 2003,pp.429-439(11) Jonnne Birchall. Sampling and samples Market Research World. Online article: accessed on 15th July 2009. https://www.marketresearchworld.net. Dr.Rob C.A.A van Schie,Dr Mark E.Wilson Saliva: Your Spitting Image National Institutes of Health in Bethesda, Maryland. October 1997. Online article: accessed on 15th July 2009.https://www.nih.gov/news/pr/oct97/nidr-22.htm

Nestle Business Ethics - 1126 Words

Professional ethics can be described as a system which is put in place to keep and order and honestly in the workplace. Ethics in the a professional setting help employees and employers perform their job duties with principle and dignity. Professional ethics help set a guideline for the workplace behavior expected from all members of the workforce. Below are a few examples of unethical practices performed by professional organizations. Nestlà © is a Swiss food and drink company. The company’s products include cereal, coffee, tea, water, and other food and drink products. Nestlà © is a very well-known and successful brand. It was reported that 29 of their brands currently have sales of over one billion dollars annually, and it has around†¦show more content†¦The chairman of Nestlà ©, Peter Brabeck-Letmathe, stated that he believes that human beings’ having a right to access water is considered an opinion and not a fact. After public backlash over this statement, Brabeck-Letmathe retracted his statement and voices his false concern for advocating access to safe water for the world’s population. Nestlà © has been getting its water for its bottled water from California, even though Nestlà ©Ã¢â‚¬â„¢s permit to do this job expired in 1988. The company is able to continue with this unethical practice by paying a very immaterial annual fee of around $500. This practice is even more of an ethical issue because Nestlà © continues its operations despite the fact that California is experiencing severe droughts. In other news, Nestlà © has also been under litigation for mislabeling its bottled water, which in reality is just bottled tap water. Other unethical practices by the Nestlà © Company include promotion of child labor and various health threats in small children. Nestlà © is known to purchase its cocoa beans from areas that promote child labor. 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Important scene to perform Essay Example For Students

Important scene to perform Essay The explorative strategies we used were physical theatre when I was being the door, and role play when Stephanie Orford took on the role of Mr Kipps. Physical theatre is when you tell the story through physical terms, and role play is when you take on somebody elses thoughts, feelings and emotions. Physical theatre helped me to understand my role and the play, for I saw how being things like a door could create tension by showing something is behind it. It also helped me understand how emotionally and physically scared Mr Kipps would have felt when he kept on hearing the banging. For the banging on the door, we decided to use a Red spot light, which started to flash on and off, to the rhythm of the banging on the door. We did this to represent Mr Kipps heartbeat, and to represent something evil and dangerous behind the door. The spotlight then went a dim white, to represent nothing, and to show the banging had stopped. This would calm the audience down, and make their tension go even higher when we started the banging again, for the white spot light would make them think there was nothing there. This scene was meant to represent the bedroom in Eel Marsh house, and how terrified Mr Kipps would have felt to be there on his own. In the next scene, we did exactly the same, and used the same explorative strategies, and everybody was in the same place, apart from this time it was Stephanie Charles who tried to open the door, and this time I did actually open, but slammed shut again. Once again we used the flashing , Red lighting to create tension and suspense in the audience. Sophie Moore still did the banging, and Stephanie Orford was not in this scene. We swapped the roles around, so everybody would have something to do, for we had too much people for that scene in the play. I do not think we should have done this though, because it would have made the audience confused. In this scene, we still thought it would be best that Sophie did the banging for me, so again we did not spoil the tension, for they would not know where it was coming from. At this point, Stephanie Charles, was walking along like Stephanie Orford very slowly and cautiously again, but this time she looked a lot more frightened than Stephanie Orford, for the banging had started again. This would have made the tension in the audience rise up even higher at a quicker pace, for they would not be expecting it, from the white spot light in the last scene that represented nothing. As she got closer to the door handle, we decided to make the banging louder and louder, to make the audience want to keep watching for they would wonder what was there, and at the same time to make them feel really frightened and shocked. This time we also decided to make Stephanie Charles reach for the door handle in an even more slower pace than Stephanie Orford did, for this would keep the audience watching and would make the tension build up higher for every second she reached for the door handle. Then to build the tension even higher, she turned the door handle even more slower, which would really keep them watching, and would make them emotionally scared and worried for Stephanie Charles. When Stephanie Charles actually did open the door, we decided to have nothing in there, to make the audience want to keep watching to see where this mysterious banging was coming from, and who was doing it. If we did have the woman in black in there, it would spoil the tension for the audience would know what to expect. By having nothing in there makes it a mystery. The explorative strategies we used was physical theatre and role play again. The role play helped me to understand how terrified Mr Kipps must of felt when he was inside the nursery room, and how it felt to have a presence with you. Physical theatre helped me to understand the fact that I was the one creating most of the tension in our performance, for in the play the banging was the rocking chair coming from behind the nursery door, and I was being the nursery room door. Once again we used a Red flashing light to represent Mr Kipps heartbeat and to go with the rythym of the banging. We thought it was important for the lighting to go with the rhythm of the banging, otherwise it would look a bit muddled up and confusing. When the door opened, I think we should have had a long, loud scream to represent the haunting of the woman in black and the death of Stella and her child on the pony and trap, for this would make the audience really scared and shocked. We could have also made the lighting a still orange, to show the creepiness and horror Mr Kipps would have felt. Red would have also been good to show these emotions, but the performance would have been more interesting with a range of colours. We decided to have the door slam shut to really make the audience startle, but would have been better if Sophie bung a bit louder, and I if emphasised it a lot better. For this, we decided to have all the lighting go completely out, to make the audience really scared, startled and worried, for they would not know what sort of presence was there with them. In 1968, a man called Franco Zefferelli produced a film of the Shakespeare classic EssayThe explorative strategies we used in this scene was still image and role play. We used still image when we were all bent over Stephanie Orphord, because we wanted to show the most important emotions of the woman in black and Mr Kipps, so the audience could understand the story and emotions of Mr Kipps. It also creates tension. The role play helped Stephanie Orford to understand emotions of Mr Kipps, and it helped us to understand why the woman in black was haunting him. It helped us to understand the woman in blacks life story and why it was so bad for her. We decided to use Orange lighting again for when we stood over Mr Kipps, for once again it created an eerie atmosphere and was good at displaying emotions. It was also good for making our white masks stand out and good for showing the kind of tension between the woman in black and Mr Kipps. Evaluation  In the actual performance of the Woman in Black, I could see lots of different drama elemants being used.  Explorative strategies used in the woman in Black stage play.  I noticed thought tracking for when Mr Kipps was reading out the letters at Eel Marsh house which he got out of a large basket. As he read the letters in his head, a recorded voice played out loud to represent the person who wrote them, who was Jennet Humfrye. The effect this element had on the audience was that it revealed Jennet Humfryes inner thoughts to them, and makes the audience feel more involved by the drama being deepened. The audience also understood the character of the woman in black, and what her past life was like, and why she ended up becoming mad. It also marked Eel Marsh house as a really important place to the woman in black, and why she returns to this place so often. The lighting for this scene was very dim, but not completely dark. Because the light was not completely dark, it was really good for showing Mr Kipps thoughts and emotions as he read the letters, and finally found out the background to the woman in black. The dim light also makes the audience feel relaxed and calm, but at the same time a little unsafe, because it makes the scene look creepy. This also relates to the creepiness of Eel Marsh House, and the amount of hauntings it recieves from the woman in black. It also relates to the fact that the nursery is one of the most important rooms in the play, and how it would have been in real life when the woman in black actually did die. The nursery room would have brought back alot of memories to the woman in black about her child, and how her son grew up there. The recorded voice also had a slight echoe to it, which marks this voice as an important voice which has a lost connection with the child, Nathaniel. It also gives the voice a spooky quality which creates an eery atmosphere in the audience. The tone of this voice sounded quite sharp, angry and lonely at the same time. These emotions are really suitable for Jennet Humfrye, for not being able to have a proper connection with her son would of made her feel isolated from the people she really cared about, but knows her son will never know that she is really his mother. The anger and sharpness of her voice would represent how angry she is at Mrs Drablow for taking her place as a mother, and for Mrs Drablow trying to bring Nathaniel up as her own. In my performance with Sarah Butters, Adelade Jones and Emily Broad, we used thought tracking at the end, when me and Adelade were pretending to be Mr Kipps and the actor. Sarah used thought tracking to represent the tension between me and Adelade, and the anxiety and terror I felt when we spoke of the woman in black. We used thought tracking, because we thought it was effective in relating to the connection between Mr Kipps and the woman in black, and how the woman in black effected Mr Kipps sons life. This scene also shows how Arthur Kipps cannot bear to bring up his child, for his death reminds him of who caused it. We also used physical theatre in this scene, for Emily and Sarah were pretending to be coat rails. We made this obvious by hanging scarves on their arms. This looked effective for it made interesting use of a normal household object. It also helped Emily to switch to the role of the woman in black really quickly again. Mr Kipps looks quite nervous talking about the woman in black, and walks away quickly, which relates to how the woman in black affected his family and left him alone.